Xylose Lysine Deoxycholate Agar | Culture Media (2024)

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SKU No. 700003040 | Catalog No. NCM0027A Related Products Documents Support Get in Touch Validation You may also like

Xylose Lysine Deoxycholate (XLD) Agar

SKU No. 700003040 |Catalog No. NCM0027A

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Xylose Lysine Deoxycholate (XLD) Agar

Xylose Lysine Deoxycholate Agar | Culture Media (1)

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SKU No. 700003040 | Catalog No. NCM0027A

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Xylose Lysine Deoxycholate Agar is used for the isolation and differentiation of enteric pathogens. Conforms to Harmonized USP/EP/JP Requirements. XLD Agar is not intended for use in the diagnosis of disease or other conditions in humans.

A medium recommended by the Harmonized Pharmacopeia for isolation and identification of Salmonella from non-sterile products. Conforms to USP/EP/JP performance specification. Originally formulated by Taylor to differentiate enteric pathogens, the agar is widely used as the preferred differential medium for Salmonella spp. The medium is void of peptones but instead uses yeast extract as a carbon, nitrogen and vitamin source and xylose, lactose and sucrose are fermentable carbohydrates. Salmonella are able to ferment xylose to produce acid but not lactose or sucrose. When the xylose is exhausted Salmonella will decarboxylate lysine shifting the pH back to neutral. At near neutral pH, Salmonella can reduce sodium thiosulfate producing hydrogen sulfide which creates a complex with ferric ammonium citrate to produce black or black centered colonies. Other organisms are able to decarboxylate lysine but acid production from the fermentation of lactose and sucrose keeps the pH too acidic for H2S production. Selectivity is achieved through the incorporation of sodium deoxycholate and phenol red acts as a pH indicator. According to the Harmonized Pharmacopeia, Rappaport Vassiliadis Salmonella Enrichment Broth (NCM0103) is used as a selective enrichment broth, with subculture performed onto Xylose Lysine Deoxycholate (XLD) agar.

Specifications
BrandNeogen® Culture Media
AnalyteSalmonella spp.
PlatformDehydrated Culture Media
Mass500 g
Formula
FormulaLiter
Xylose3.5 g/L
L-Lysine5.0 g/L
Lactose Monohydrate7.5 g/L
Sucrose7.5 g/L
Sodium Chloride5.0 g/L
Yeast Extract3.0 g/L
Phenol Red0.08 g/L
Agar13.5 g/L
Sodium Deoxycholate2.5 g/L
Sodium Thiosulfate6.8 g/L
Ferric Ammonium Citrate0.8 g/L
Grams per Liter55.00
Liters per 500 g9.00
Plates per 500 g500.00
Preparation
  1. Suspend 55 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation until the medium reaches the boiling point.
  3. Avoid overheating. DO NOT AUTOCLAVE.
  4. Cool to 45-50°C.
  5. Pour into plates as soon as the medium has cooled.
  6. Protracted boiling or prolonged holding at elevated temperature induces precipitation.
Previous Item NumberProduct was previously known as Acumedia Product: 7166 XLD Agar; and LabM Product: HP008 Xylose Lysine Deoxycholate Agar (USP/EP/JP)
Package Dimensions4.60 in x 2.90 in x 8.60 in
Package Weight1.35 lb

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Technical Specification Sheet

Xylose Lysine Deoxycholate (XLD) Agar

Safety Data Sheet (SDS)

XLD Agar - English - US XLD Agar - Bulgarian - Bulgaria XLD Agar - Chinese Simplified - China XLD Agar - Chinese Traditional - Taiwan XLD Agar - Croatian - Croatia XLD Agar - Czech - Czech Republic XLD Agar - Danish - Denmark XLD Agar - Dutch - Belgium XLD Agar - Dutch - Netherlands XLD Agar - English - Australia XLD Agar - English - Canada XLD Agar - English - EU/UK XLD Agar - English - Ireland XLD Agar - English - Malta XLD Agar - English - New Zealand XLD Agar - English - Philippines XLD Agar - Estonian - Estonia XLD Agar - Finnish - Finland XLD Agar - French XLD Agar - French - Belgium XLD Agar - French - Canada XLD Agar - German XLD Agar - German - Austria XLD Agar - German - Luxembourg XLD Agar - Greek - Cyprus XLD Agar - Greek - Greece XLD Agar - Hungarian - Hungary XLD Agar - Indonesian - Indonesia XLD Agar - Italian - Italy XLD Agar - Japanese - Japan XLD Agar - Korean - Korea XLD Agar - Latvian - Latvia XLD Agar - Lithuanian - Lithuania XLD Agar - Malay - Malaysia XLD Agar - Maltese - Malta XLD Agar - Norwegian - Norway XLD Agar - Polish - Poland XLD Agar - Portuguese - Brazil XLD Agar - Portuguese - Portugal XLD Agar - Romanian - Romania XLD Agar - Slovak - Slovakia XLD Agar - Slovene - Slovenia XLD Agar - Spanish - Colombia XLD Agar - Spanish - Mexico XLD Agar - Spanish - Spain XLD Agar - Spanish - US XLD Agar - Swedish - Sweden XLD Agar - Thai - Thailand XLD Agar - Vietnamese - Vietnam

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Xylose Lysine Deoxycholate Agar | Culture Media (2024)
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